Chromatin Higher-order Structure and Dynamics

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Chromatin higher-order structure and dynamics.

The primary role of the nucleus as an information storage, retrieval, and replication site requires the physical organization and compaction of meters of DNA. Although it has been clear for many years that nucleosomes constitute the first level of chromatin compaction, this contributes a relatively small fraction of the condensation needed to fit the typical genome into an interphase nucleus or...

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Methods for identifying higher-order chromatin structure.

Eukaryotic genomic DNA is combined with histones, nonhistone proteins, and RNA to form chromatin, which is extensively packaged hierarchically to fit inside a cell's nucleus. The nucleosome-comprising a histone octamer with 147 base pairs of DNA wrapped around it-is the initial level and the repeating unit of chromatin packaging, which electron microscopy first made visible to the human eye as ...

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Higher-order chromatin structure: bridging physics and biology.

Advances in microscopy and genomic techniques have provided new insight into spatial chromatin organization inside of the nucleus. In particular, chromosome conformation capture data has highlighted the relevance of polymer physics for high-order chromatin organization. In this context, we review basic polymer states, discuss how an appropriate polymer model can be determined from experimental ...

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Chromatin Higher Order Structure and Chromosomal Positioning in the Nucleus

While it has been known for some time that chromatin is highly packaged, it has only recently been discovered that chromatin is attached to the nuclear matrix. It has also been recently shown that interphase chromosomes remain separate from one another in so called, “chromosome domains.” This author suggests a functional relationship between chromatin attachment to the nuclear matrix and chromo...

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The Drosophila Mi-2 Chromatin-Remodeling Factor Regulates Higher-Order Chromatin Structure and Cohesin Dynamics In Vivo

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ژورنال

عنوان ژورنال: Cold Spring Harbor Perspectives in Biology

سال: 2010

ISSN: 1943-0264

DOI: 10.1101/cshperspect.a000596